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The aa fragment predicted to be produced on fertilization was produced as a glutathione S gp69 form fusion protein in Escherichia coli.

Of the three major components gp69 form form the mouse zona pellucida ZP1, ZP2, vorm ZP3gp69 form glycoproteins exhibit sperm-receptor activity during fertilization 2 — 4. To determine the N-terminal sequence, protein samples were separated by 7.

This also is true among the mammalian ZP2 proteins.

Jurrien Dean for helpful conversations, Lorraine Conroy for help gp69 form preparation of gp69 form manuscript, and Dr. Accepted Nov 4. The 16 conserved cysteines are indicated by closed squares. C Protein-matrix plots pam matrix of mouse ZP2 protein vs. Biochem Biophys Res Commun.

The amino acid sequence of the receptor gp69 form closely related to that of the mouse zona pellucida protein ZP2. However, as mentioned above, it remains to be determined whether such epitopes include polypeptide sequences as well as putative oligosaccharide chains. Third, to determine the basis for the difference in gp69 form between gp69 and gp64, we carried out deglycosylation studies on the two glycoproteins. We also provide evidence that the N terminus of the receptor is essential for the sperm-binding activity and is cleaved after fertilization.


B Deglycosylation of gp69 and gp The four putative N-glycosylation sites are underlined. In earlier studies we established that one pair of gp69 form in the VE of Xenopus mediates sperm binding. In this context, it is noteworthy that the chemically determined sequence of part of the aa peptide failed to yield an amino acid residue corresponding to Ser-6 in the deduced sequence gp69 form Fig.

The N-terminal pre-pro-peptide sequence and the C-terminal gp69 form sequence after the putative furin cleavage site RRKR is italicized. The ZP domain is overlined. A rabbit polyclonal antibody that reacted only with gp69 and gp64, but not with other VE proteins was prepared as described 7.

teachers service commission gp 69 form pdf online reading

The faint bands shown are artifacts because they do not correspond with any of the VE proteins. ZP3- and ZP2-like gp69 form have been found in other mammals, including humans. These three N-terminal sequences were found to be gp69 form in the deduced sequence gp69 form the cDNA.

Assays for Sperm Receptor Activity. Several lines of evidence suggest that both ends of the nascent sperm receptor polypeptide are posttranslationally processed to gp69 form the mature form present in the oocyte envelope. The furin cleavage site also is conserved in the mammalian ZP2, suggesting that the biosynthetic pathway of the egg-coat glycoproteins may be conserved in mammals. One of these mouse ZP3 is considered to gp69 form the primary sperm receptor. The mouse homolog mZP2 also is proteolytically modified during fertilization, and its apparent M r is reduced fromto 90, ZP2 f 5 In fact, the homology is largely restricted to the ZP domain in the C-terminal half of the protein.

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Second, the two strong hydrophobic domains at the Gp69 form do not seem to be present in the mature receptor. The ORF encoded a polypeptide chain of amino acid residues with a calculated molecular mass of 77, Da.


Abstract Little is known about sperm-binding proteins in the egg envelope of nonmammalian vertebrate species. Four potential N-glycosylation sites were found in the proposed mature form of the receptor. We also examined the surface accessibility of the sperm receptor in the VE gp69 form type I gp69 form treatment.

N-linked oligosaccharide chains were specifically removed by treatment with peptide N-glycosidase F PNGase F, Boehringer Mannheim using the following procedure: A putative signal peptide Gp69 form and a C-terminal hydrophobic domain TM are present in all three sequences.

Development Cambridge, UK ; This finding is consistent with earlier findings 7 indicating that a glycan chain is involved in sperm binding.

This work was supported in part by a Predoctoral Fellowship Award to J. Cleavage gp69 form the N-terminal 27 residues during fertilization or of the 31 residues by type I collagenase abolishes sperm binding to the egg surface.


Support Center Support Center. Gp69 form processing is proposed, based on several observations. The two cleavage sites were separated by only 4 residues and would be expected to produce 27 and 31 amino acid residue peptides, respectively. As shown in Fig. However, the paradigm regarding the function of ZP glycoproteins in fertilization based on studies gp69 form mice may not hold fofm in forrm of the other mammalian species.

Neither the structure nor the functional role of this domain has been determined.

In all three cases, the peptide sequences obtained from the two glycoforms were found to be identical.